| Gene | Primer name | Sequence (5’–3’) | Product (bp) |
| D-alanine:D-alanine ligase (ddl) | ddlF | ATCAAGTACAGTTAGTCTT | 941 |
| ddlR | ACGATTCAAAGCTAACTG | ||
| RAPD Primer | D8635 | GAG CGG CCA AAG GGA GCA GAC | |
| Aggregation substance (asa+) | ASA 11 | GCACGCTATTACGAACTATGA | 375 |
| ASA 12 | TAAGAAAGAACATCACCACGA | ||
| Gelatinase (gel+) | GEL 11 | TATGACAATGCTTTTTGGGAT | 213 |
| GEL 12 | AGATGCACCCGAAATAATATA | ||
| Cytolysin (cyl+) | CYT I | ACTCGGGGATTGATAGGC | 688 |
| CYT IIb | GCTGCTAAAGCTGCGCTT | ||
| Enterococcal surface protein (esp+) | ESP 14F | AGATTTCATCTTTGATTCTTGG | 510 |
| ESP 12R | AATTGATTCTTTAGCATCTGG |